Infraspecific morphological and molecular variation of Linum nervosum (Linaceae) in Iran

  • Maryam Rashnou-Taei Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Velenjak, 1983969411 Tehran, Iran
  • Masoud Sheidai Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Velenjak, 1983969411 Tehran, Iran
  • Seyed Mehdi Talebi Department of Biology, Faculty of Sciences, Arak University, Sardasht, 3815688349 Arak, Iran
  • Zahra Noormohammadi Department of Biology, Science and Research Branch, Islamic Azad University, Simon blvd., 1477893855 Tehran, Iran
  • Fahimeh Koohdar Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Velenjak, 1983969411 Tehran, Iran

Abstract

Linum nervosum is among species that can hybridize with L. usitatissimum and produce fertile offsprings. Genetic diversity analysis of this wild relative of flax is important from conservation and breeding points of view. In the present study, 55 randomly selected plants of six different populations of L. nervosum varieties, including var. nervosum and var. bungei, were studied for morphological and genetic variability as well as population structure. Analysis of variance (ANOVA) did not show significant morphological difference between populations. PCA as well as PCA biplot confirmed that some morphological traits have taxonomic value. UPGMA clustering separated the populations of varieties in two distinct clusters, indicating degrees of morphological differentiation between them. Furthermore, UPGMA confirmed the variability in morphological characters within populations. Neighbor Joining tree and Neighbor-Net analysis of ISSR data revealed inter- and intrapopulation genetic variability. STRUCTURE plot revealed allelic difference of these varieties and some degree of intervarietal gene flow. K-means clustering showed the fragmentation of populations in support of AMOVA test, which revealed significant genetic difference among them. In general, obtained results confirmed the alternation of taxonomic level of L. bungei to the variety of L. nervosum.

References

Caliński R.B., Harabasz J. 1974. A dendrite method for cluster analysis. Commun. Stat. 3: 1–27. https://dx.doi.org/10.1080/03610927408827101
Catlow A. 2012. Popular garden botany: Containing a familiar and scientific description of most of the hardy and half-hardy plants introduced into the flower garden. Nabu Press, United States.
Cullis C. 2011. Linum. In: Kole C. (ed.), Wild crop relatives: Genomic and breeding resources, oilseeds. Springer-Verlag, Berlin Heidelberg.
Dzybov D. 2013. Agrosteppe method. Env. Nat. Resour. Res. 3 (3): 71–77. https://dx.doi.org/10.5539/enrr.v3n3p71
Egorova T.V. 2000. Taxonomic review of the genus Linum (Linaceae) in the Caucasian flora. Bot. Zhurn. 85 (7): 164–176. (In Russian)
Falush D., Stephens M., Pritchard J.K. 2007. Inference of population structure using multilocus genotype data: Dominant markers and null alleles. Mol. Ecol. Notes 7 (4): 574–578.
Freeland J.R., Kirk H., Peterson S.D. 2011. Molecular Ecology, 2nd ed. Wiley-Blackwell, UK.
Frichot E., Schoville S.D., Bouchard G., Francois O. 2013. Testing for associations between loci and environmental gradients using latent factor mixed models. Mol. Ecol. Notes 30: 1687–1699. https://doi.org/10.1093/molbev/mst063
Futuyma D.J., Mayer G.C. 1980. Non-allopatric speciation in animals. Syst. Zool. 29: 254–271.https://doi.org/10.2307/2412661
Gavrilets S. 2004. Fitness landscapes and the origin of species. Princeton University Press, Princeton, NJ.
Hamer Ø., Harper D.A.T., Ryan P.D. 2012. PAST: Paleontological Statistics software package for education and data analysis. Palaeontol. Electron. 4 (1): 1–9.
Hedrick P.W. 2005. A standardized genetic differentiation measure. Evol. 59: 1633–1638. https://doi.org/10.1111/j.0014-3820.2005.tb01814.x
Hickey M. 1988. 100 families of flowering plants, 2nd ed. Cambridge University Press, Cambridge.
Holsinger K.E., Lewis P.O. 2003. Hickory: A package for analysis of population genetic data V. 1.0. Accessed from http://www.eeb.uconn.edu
Huson D.H., Bryant D. 2006. Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23: 254–267. https://doi.org/10.1093/molbev/msj030
Jhala A.J., Hall L., Hall M., Jocelyn C. 2008. Potential hybridization of flax with weedy and wild relatives: An avenue for movement of engineered genes? Crop Sci. 48: 825–840. https://doi.org/10.2135/cropsci2007.09.0497
Jost L. 2008. GST and its relatives do not measure differentiation. Mol. Ecol. 17 (18): 4015–4026. https://doi.org/10.1111/j.1365-294X.2008.03887.x
Križman M., Jakse J., Baricevic D., Javornik B., Prošek M. 2006. Robust CTAB-activated charcoal protocol for plant DNA extraction. Acta Agric. Slov. 87 (2): 427–433.
Meirmans P.G., Van Tienderen P.H. 2004. GENOTYPE and GENODIVE: Two programs for the analysis of genetic diversity of asexual organisms. Mol. Ecol. Notes 4: 792–794. https://doi.org/10.1111/j.1471-8286.2004.00770.x
Muir A.D., Westcott N.D. (eds) 2003. Flax: The genus Linum. Taylor and Francis Inc., London, New York.
Ockendon D.J. 1971. Taxonomy of the Linum perenne group in Europe. Watsonia 8: 205–235.
Peakall R., Smouse P.E. 2006. GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Mol. Ecol. Notes 6: 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
Podani J. 2000. Introduction to the exploration of multivariate biological data. Backhuys Publishers, Leiden.
Pritchard J.K., Stephens M., Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics 155: 945–959.
Rechinger K.H. 1974. Flora Iranica. no. 106. Linaceae. Akademische druck-u. Verlag Sanstalt, Graz, Austria.
Samadi A., Mahmoozadeh A., Hassanzadeh-Gorttapeh A., Torkamani M.R. 2007. Cytogenetic studies in four species of Flax (Linum spp.). J. Appl. Sci. 7: 2832–2839. https://dx.doi.org/10.3923/jas.2007.2832.2839
Schwarz G. 1978. Estimating the dimension of a model. Ann. Stat. 6: 461–464. http://www.jstor.org/stable/2958889
Sharifnia F., Assadi M. 2001. Flora of Iran. No. 34. Linaceae. Ministry of Jahad-e-Sazandegi, Research Institute of Forests and Rangelands, Iran.
Sheidai M., Afshar F., Keshavarzi M., Talebi S.M., Noormohammadi Z., Shafaf T. 2014a. Genetic diversity and genome size variability in Linum austriacum (Linaceae) populations. Biochem. Syst. Ecol. 57: 20–26. https://dx.doi.org/10.2478/s11756-013-0281-4
Sheidai M., Farahani F., Talebi S.M., Noormohammadi Z. 2015. Genetic and morphological analysis of distyly in Linum mucronatum Bertol. (Linaceae). Phytol. Balc. 21 (1): 13–19.
Sheidai M., Ziaee S., Farahani F., Talebi S.M., Noormohammadi Z., Hasheminejad-Ahangarani Farahani S. 2014b. Infra-specific genetic and morphological diversity in Linum album (Linaceae). Biologia (Bratislava) 69: 32–39. https://doi.org/10.2478/s11756-013-0281-4
Talebi S. M., Farahani F., Sheidai M., Noormohammadi Z. 2014. Palynological characteristics of the heterostylous subspecies of Linum mucronatum Bertol. Collect. Bot. 33: e004. https://doi.org/10.3989/collectbot.2013.v33.004
Talebi S.M., Sheidai M., Atri M., Sharifnia F., Noormohammadi Z. 2012. Genome size, morphological and palynological variations, and heterostyly in some species of the genus Linum L. (Linaceae) in Iran. Afr. J. Biotechnol. 11 (94): 16040–16054.
Tero N., Aspi J., Siikamaki P., Jakalaniemi A., Tuomi J. 2003. Genetic structure and gene flow in a metapopulation of an endangered plant species Silene tatarica. Mol. Ecol. 12: 2073–2085. https://doi.org/10.1046/j.1365-294X.2003.01898.x
Weising K., Nybom H., Wolff K., Kahl G. 2005. DNA Fingerprinting in plants, principles, methods, and applications, 2nd ed. Taylor and Francis.
Zolotukhin N., Zolotukhin I., Poluyanov A., Filatova T. 2014. Natural conditions of the Streletsky steppe (Kursk region). The 11th European dry grassland meeting "Steppes and semi-natural dry grasslands: Ecology, transformation and restoration": 89–97. Tula, Russia.
Figure 1. General view of Linum nervosum with demonstration of some measured features.
Published
2018-05-26
How to Cite
RASHNOU-TAEI, Maryam et al. Infraspecific morphological and molecular variation of Linum nervosum (Linaceae) in Iran. Modern Phytomorphology, [S.l.], v. 12, p. 43-58, may 2018. ISSN 2227-9555. Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/516>. Date accessed: 26 aug. 2018. doi: https://doi.org/10.5281/zenodo.1253614.
Section
Research Articles