Identification and assay of the flavonoids in medicinal plants with hepatoprotective action

  • Maria A. Cojocaru-Toma State University of Medicine and Pharmacy „NicolaeTestemițanu”, Department of Pharmacognosy and Pharmaceutical Botanic, Malina Mică str. 66, Chișinău MD 2025, Republic of Moldova

Abstract

The article describes the identification and assay of the flavonoids in medicinal plants with hepatoprotective action, harvested as a culture at the Cultivation Center of the Medicinal Plants within State University of Medicine and Pharmacy „Nicolae Testemițanu” from the Republic of Moldova, using Pharmacopoeia methods. The flavonoids, found in the examined medicinal product, are responsible for hepatoprotective activity due to antioxidant activity, exhibited by neutralizing free radicals. The flavonoids were identified by using the Chinode method and thin layer chromatography, operating with 3 systems of solvents, and as biomarkers rutine, quercetine and luteolin ewere used. The results of the quantitative analysis point out that the content of the flavonoids determined by using spectrophotometric method is in the range of 0.620% to 1.204%, in studied vegetal products.

References

Cojocaru-Toma M. 2014. Valorificarea plantelor medicinale cu acțiune hepatoprotectoare din flora Republicii Moldova. Buletinul Academiei de științe 1 (42): 237–341.
Ciulei I., Istudor V., Palade M., Albulescu D., Gоrd C. 1995. Analiza farmacognostică și fitochimică a produselor vegetale. Vol. I: 22–46. Ed. Tehnoplast Company, București.
European Pharmacopoeia. 2014. Vols I and II. 8th ed.
Negru A. 2007. Determinator de plante din Flora Republicii Moldova: 305–311. Chișinăup.
Onigă I., Benedec D., Hanganu D. 2004. Analiza produselor naturale medicinale Universitatea de Medicină şi farmacie Iuliu Haţineanu: 94-104. Cluj – Napoca.
Programul național decombatere a hepatitelor virale B, C și D în Moldova pentru anii 2012-2016. Monitorul Oficial al Republicii Moldova Nr. 34-37 din 17.02.2012.
Fig. 1. Identification of flavonoids by thin-layer chromatografy, a and b systems.
Published
2015-05-20
How to Cite
COJOCARU-TOMA, Maria A.. Identification and assay of the flavonoids in medicinal plants with hepatoprotective action. Modern Phytomorphology, [S.l.], v. 8, p. 61-64, may 2015. ISSN 2227-9555. Available at: <http://ojs.phytomorphology.org/index.php/MP/article/view/58>. Date accessed: 11 apr. 2018. doi: https://doi.org/10.5281/zenodo.159832.
Section
Correspondences